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Re: Yet another h.pylori infectee

Posted: Thu Sep 03, 2015 10:50 pm
by sparklypickle
Test came back negative (one week after end of ab course)....so....not sure where that leaves me. I'm feeling slightly less dreadful today so hopefully that means that I'm on the mend? Time to bring out the valium and probiotics I think.

Re: Yet another h.pylori infectee

Posted: Fri Sep 04, 2015 9:36 am
by Helico_expert
Congratulation! I think you just need to gather positive thoughts and give your stomach some time to heal.
:)

Re: Yet another h.pylori infectee

Posted: Fri Sep 04, 2015 2:14 pm
by sparklypickle
Thanks! Having a positive voice of support really makes a difference :) did you see my Scientific questions in the last post?

Re: Yet another h.pylori infectee

Posted: Mon Sep 07, 2015 1:18 pm
by Helico_expert
Hi,

sorry i missed your questions.

In terms of work related antibiotic resistance, I think that depends on your aseptic technique. If you did it correctly, you should be contaminated with laboratory antibiotics induced resistance.

In terms of iron deficiency, H. pylori is associate with low iron deficiency. Some patients' iron level return to normal after eradication of H. pylori. The mechanism is unknown, but it works for some people.

in terms of antibiotic resistance pattern, each country is different. In Australia, most of our strains are resistant to Clarithromycin (>90%) and Metronidazole (>60%). In China, the Metronidazole resistance is as high as 90%.
read our paper, there is a table display resistance in Australia
http://onlinelibrary.wiley.com/doi/10.1 ... 12089/full

Read this paper, there is a table display reistance in China
http://onlinelibrary.wiley.com/doi/10.1 ... 12046/full

In terms of tetracycline, we havent really seen one strain that has resistance to Tet. This antibiotic targets the 16s rRNA. The gene is probably too conserve and too costly to mutate. There are reports of Tetracycline resistant H. pylori strains. But we have not seen one.

Re: Yet another h.pylori infectee

Posted: Mon Sep 07, 2015 3:40 pm
by sparklypickle
Thanks helico_expert :) that's interesting. I will have a look at those papers.

I think my sterile technique is good because I very rarely get contamination when working without antibiotics, I just wondered if in general there were more ab resistant strains kicking about in a lab.

Ooh the fact that tetracycline affects the ribosome brings me to another question...how is helicobacter able to mutate so much faster than most other bacterial strains? Does It have really poor proofreading in its DNA polymerase?

Re: Yet another h.pylori infectee

Posted: Tue Sep 08, 2015 10:01 am
by Helico_expert
In the lab, you should be constantly spraying alcohol to avoid contamination of bacteria. Nevertheless, even the top rank laboratories are spreading HeLa cells everywhere. read the story here
http://berkeleysciencereview.com/article/good-bad-hela/

in regards to H. pylori's mutation, yes, H. pylori lack of the proof reading function in the polymerase. So every time it multiply, it makes a little error. We sequenced the genome of H. pylori of 3 passages and there, accumulated at least 2 SNPs. However, when you put H. pylori into a new hosts (eg. Mouse), then passage in agars, you'll see over 1000 SNPs. So the mutation rate is also related to stress and environment change.

can read this paper.
http://www.nature.com/ncomms/2014/14061 ... s5165.html

Re: Yet another h.pylori infectee

Posted: Fri Nov 13, 2015 5:14 pm
by sparklypickle
Hello. My followup test at 6 weeks post treatment was negative so I started to taper off omeprazole and the prebiotic I had started taking. Starting from 40mg daily Omeprazole at 20mg.per day was fine, 20mg every other day was a bit suboptimal but leaving it three or four days between doses is horrible.The problem is, I'm now having a horrible sensation in my stomach and upper intestines like they are constantly burning or something. Antacids do nothing to relieve it. I've also reverted to my previous bowel habits of loose stools in the morning and hard ones at night...neither are normal and I feel kind of queasy. I was up at 3am with crippling gas pains last night too :( I will go to the Dr next week to discuss because I feel like my digestive system is really unhappy about something. I still can't look at orange juice, coffee or alcohol.

Re: Yet another h.pylori infectee

Posted: Wed Nov 18, 2015 3:49 pm
by Helico_expert
hi, just want to make sure that when you did your followup test, you are not taking any PPI or antibiotics for at least 2 weeks. Any drugs that reduce your stomach acid will give you a negative breath/stool test.

Nevertheless, assuming that you did the test correctly and truly now free of H. pylori, perhaps you need to speak to a dietitian or immulogist to check if you are allergy to certain food.

Re: Yet another h.pylori infectee

Posted: Thu Nov 19, 2015 3:09 pm
by sparklypickle
Hi, I only stopped ppi for two days as that is what my Dr advised, but it was 6 weeks after abs. I've been told to continue ppis for the foreseeable future because my stomach is still overproducing acid, and Dr thinks I have ibs...I'm being referred to a dietician and also for counselling as it is very likely due to anxiety. I think my helicobacter journey is over but it's just annoying that my stomach is not how it used to be despite an apparent eradication. If I ever manage to come off omeprazole then I may ask for anothr test to be sure.

Re: Yet another h.pylori infectee

Posted: Thu Nov 19, 2015 3:58 pm
by Helico_expert
there are many ways of stopping acid production. Some has strong and long lasting effect and some have shorter effect. For people who needs to do UBT or stool test, it is better to use the short effect life drugs.

Please refer to the reference table from the link below to choose your acid inhibitor before your next testing.
http://www.helico.com/PYtest_guide_2013.pdf